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Chromatography is a kind of mixture separation technology and has a wide range of applications in analytical chemistry, organic chemistry, biochemistry and other fields. Chromatography utilizes the differences in the physical and chemical properties of each component in the mixture to separate and determine the multi-component mixture. According to the chromatography mechanism, it can be divided into adsorption chromatography, partition chromatography, ion exchange chromatography, gel filtration chromatography, etc.
Specific applications of chromatography include
Desalting For example, gel chromatography is used to remove low molecular weight impurities in the solution of macromolecules (such as proteins, nucleic acids, polysaccharides, etc.)
Separation and purification for the separation and purification of enzymes, proteins, polysaccharides, hormones, alkaloids and other substances
Concentration of dilute solutions
Determination of molecular weight of polymer substances
As well as various chromatographic methods and research on the properties of fillers, etc.
In the natural state, it is limited by the permeation speed of the liquid in the chromatography column. It is necessary to use appropriate external pressure to enhance the permeation capacity of the liquid and to ensure that the liquid is not cross-contaminated. Therefore, peristaltic pumps are ideal for transporting fluids during chromatography. They are often used to control the flow rate of the mobile phase, the flow rate of the eluent, pump buffers, draw samples to be tested, etc.
Diagram The peristaltic pump is connected upstream of the chromatography column, and the mobile phase, eluent, etc., are continuously pumped into the chromatography column. The solutions after chromatography are collected in different containers according to different components.
solution
The BT100-2J peristaltic pump is mainly used in the laboratory. The speed can be adjusted manually, and it can also be automatically controlled through the external control interface. It matches the pump head of YZ1515/YZ2515. The applicable hose specifications are more and the flow range is wider.
The Rongbai peristaltic pump can provide a certain fluid pressure to make the fluid pass through the chromatography column with higher resistance (the smaller the packing pores, the greater the resistance)
Rongbai peristaltic pump has high fluid transmission accuracy, which enables the fluid to pass through the chromatography column with high flow accuracy to achieve the desired chromatography effect.
The Rongbo peristaltic pump has low shear force, which can reduce the damage to the active ingredients in the solution when it is used to pump macromolecular solutions such as proteins.
Rongbai peristaltic pump is simple and convenient to replace the hose. When transferring different solutions in the laboratory, cross-contamination between different solutions can be avoided by simply replacing the hoses that meet the hygienic level.